TRI-COLOR PROBES FOR DETECTING MULTIPLE GENE REARRANGEMENTS IN A FISH ASSAY

摘要

A probe system is provided. In some embodiments, the probe system may comprise: a first labeled probe that hybridizes to one side of a potential translocation breakpoint in a first locus; a second labeled probe that hybridizes to the other side of the potential translocation breakpoint in the first locus; a third labeled probe that hybridizes to one side of a potential translocation breakpoint in a second locus; a fourth labeled probe that hybridizes to the other side of the potential translocation breakpoint in the second locus; and a fifth labeled probe that hybridizes to both sides of the potential translocation breakpoint in either the first locus or the second locus, but not both. The fifth probe is distinguishably labeled from the first, second, third and fourth probes. Methods for detecting a chromosomal rearrangement in the first and second loci using the probe system are also provided.