TRAIL MEMBRANE-PENETRATING PEPTIDE-LIKE MUTANT MUR5, PREPARATION METHOD THEREFOR, AND APPLICATION THEREOF

摘要

The Invention provides a TRAIL cell-penetrating peptide (CPPs)-like mutant MuR5 and a preparation method and the application thereof. The amino acid sequence of the mutant is SEQ ID NO: 2. The TRAIL CPPs-like mutant selectively transforms the amino acid coding sequence of No. 114-118 of the outer fragment of the TRAIL wild-type protein cell membrane from VRERG to RRRRR, i.e., mutates valine into arginine on the 114 th coding sequence, glutamic acid into arginine on the 116 th coding sequence and glycine into arginine on the 118 th coding sequence, turning the coding sequence of N-terminal of the mutant protein into that of five arginines and making it a protein containing CPPs-like structure. Having a superior therapeutic effect on different types of tumor, the TRAIL CPPs-like mutant is a new generation of high-efficient drug for inducing tumor apoptosis of much potential.