METHOD FOR CONSTRUCTING SECOND-GENERATION SEQUENCING LIBRARY FOR DETECTION OF LOW-FREQUENCY MUTATION, AND KIT THEREFOR

摘要

The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3' end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.