The Invention provides a TRAIL cell-penetrating peptide (CPPs)-like mutant MuR6 and a preparation method and the application thereof. The amino acid sequence of the mutant is SEQ ID NO: 2. The mutant selectively transforms the amino acid coding sequence of No. 114-119 of the outer fragment of the TRAIL wild-type protein cell membrane from VRERGP to RRRRRR, i.e., mutates valine into arginine on the 114 th coding sequence, glutamic acid into arginine on the 116 th coding sequence, glycine into arginine on the 118 th coding sequence and proline into arginine on the 119 th coding sequence, turning the coding sequence of N-terminal of the mutant protein into that of six arginines and making it a protein containing CPPs-like structure. Having a superior therapeutic effect on different types of tumor, the TRAIL mutant is a new generation of high-efficient drug for inducing tumor apoptosis of much potential.
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机译：本发明提供了TRAIL细胞穿透肽（CPPs）样突变体MuR6及其制备方法和应用。该突变体的氨基酸序列为SEQ ID NO：2。该突变体选择性地将TRAIL野生型蛋白细胞膜外片段的114-119号氨基酸编码序列从VRERGP转化为RRRRRR，即突变缬氨酸在第114个编码序列上变成精氨酸，谷氨酸在第116个编码序列上变成精氨酸，甘氨酸在第118个编码序列上变成精氨酸，脯氨酸在第119个编码序列上变成精氨酸，将N端的编码序列变成将突变蛋白变成六个精氨酸的蛋白，使其成为含有CPPs样结构的蛋白。 TRAIL突变体对不同类型的肿瘤具有优异的治疗作用，是新一代的高效药物，可诱导具有巨大潜力的肿瘤细胞凋亡。
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