首页> 外国专利> POLYRGD PEPTIDES OBTAINED BY CHEMICAL AND BIOTECHNOLOGY METHODS WITH INJURY REDUCING AND/OR NEUROPROTECTIVE EFFECTS AND A KIT FOR THEIR ACTIVITY EVALUATION

POLYRGD PEPTIDES OBTAINED BY CHEMICAL AND BIOTECHNOLOGY METHODS WITH INJURY REDUCING AND/OR NEUROPROTECTIVE EFFECTS AND A KIT FOR THEIR ACTIVITY EVALUATION

机译：化学和生物技术方法获得的具有伤害减轻和/或神经保护作用的小肽，以及用于评估其活性的试剂盒

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摘要

The present invention relates to chemical compounds with injury-reducing and/or neuroprotective properties, of the general formula: A₁-H₂N-(RGD)_n-amide-A₂where n=1-10.The compounds are preferably selected from the group: RGD1; RGD2; RGD3; RGD4; AcRGDl; AcRGD3, RGD10-PM; RGD10-PS.;A subject of the invention is a method for the preparation of the compound RGD10-PS, and its derivatives, by biological means.;Moreover, a subject of the invention involves methodology for the evaluation of the injury-reducing and/or neuroprotective activity of chemical compounds on a cellular level, by in vitro analysis of six injury factors, namely glucose deprivation, sodium azide-induced inhibition of cellular respiration, acidosis induced by sodium lactate acidification at pH 6.35, excitotoxicity induced by NMDA, excitotoxicity induced by glutamic acid and excitotoxicity induced by kainic acid, in neural cells.;The present invention also includes also a setup for the evaluation of the neuroprotective activity of the aforementioned chemical compounds.

机译：本发明涉及具有减少伤害和/或神经保护特性的通式的化合物：A _{1 -H _{2 N-（RGD）_{n -酰胺-A _{2 其中n = 1-10。所述化合物优选地选自：RGD1;或RGD1。 RGD2; RGD3; RGD4; AcRGDl; AcRGD3，RGD10-PM; RGD10-PS 。;本发明的主题是通过生物学手段制备化合物RGD10-PS及其衍生物的方法。此外，本发明的主题涉及评估减轻伤害和减轻伤害的方法。通过体外对六个损伤因子的体外/ I分析，确定化合物在细胞水平上的神经保护活性，这些损伤因子包括葡萄糖剥夺，叠氮化钠诱导的细胞呼吸抑制，乳酸钠在pH 6.35酸化引起的酸中毒在神经细胞中由NMDA引起的兴奋性毒性，由谷氨酸引起的兴奋性毒性和由海藻酸引起的兴奋性毒性。本发明还包括用于评估上述化合物的神经保护活性的装置。}}}} 展开▼

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公开/公告号EP3549947A1

专利类型

公开/公告日2019-10-09

原文格式PDF

申请/专利权人 UNIWERSYTET GDANSKI;POLITECHNIKA GDANSKA;GDANSKI UNIWERSYTET MEDYCZNY;INSTYTUT BIOLOGII DOSWIADCZALNEJ IM. MARCELEGO NENCKIEGO POLSKIEJ AKADEMII NAUK;MEDVENTURES SP. Z. O. O.;PRO SCIENCE POLSKA SP. Z O.O.;
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申请/专利号EP20180000319

发明设计人 CZUPRYN ARTUR;MUCHA PIOTR;SKOWRON PIOTR;MAZURYK JAROSLAW;WISNIEWSKA MARTA;KOZINSKI KAMIL;BERESEWICZ MALGORZATA;KRUPSKA OLGA;ZALUSKA IZABELA;PALCZEWSKA - GROVES MALGORZATA;ZYLICZ - STACHULA AGNIESZKA;ZEBROWSKA JOANNA;PIOTROWSKI ARKADIUSZ;PIKULA MICHAL;SACHADYN PAWEL;JANUS LUKASZ;RODZIEWICZ-MOTOWIDLO SYLWIA;
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申请日2018-04-04

分类号C07K7/06;C07K14/78;C07K7/02;

国家 EP

入库时间 2022-08-21 12:26:54

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1. 一种具有神经保护作用的小肽及应用 [P] . 中国专利： CN109400717B . 2021.05.18

2. 一种具有神经保护作用的小肽及应用 [P] . 中国专利： CN109400717A . 2019-03-01

3. polyRGD peptides obtained by chemical and biotechnological method with antidemarcation and/or neuroprotective effect and a set for determination of their activity [P] . PL425131A1 . 2019-10-07

机译：通过化学和生物技术方法获得的具有抗分界和/或神经保护作用的polyRGD肽及其活性测定装置

4. compound or a salt thereof, prodrug, drug, fatty acid amide hydrolase inhibitor, use of the compound or prodrug, fatty acid amide hydrolase inhibition methods in a mammal, brain cell protection and / or neural cells in a mammal to prevent or treat cerebrovascular disorders, head injury or spinal cord damage in a mammal, to prevent or treat disease, to protect brain cells and / or neural cells in a mammal, of Measuring a Fatty Acid Amide Hydrolase Activity and Preventing or Treating Sleep Disorders in a Mammal, Brain-Neuroprotective and Therapeutic or Prophylactic Agents, Kit, and Process for Producing a Compound [P] . BRPI0517753A . 2008-10-21

机译：化合物或其盐，前药，药物，脂肪酸酰胺水解酶抑制剂，在哺乳动物中使用该化合物或前药，脂肪酸酰胺水解酶抑制方法，在哺乳动物中保护脑细胞和/或神经细胞以预防或治疗脑血管疾病，以预防或治疗哺乳动物的疾病，保护哺乳动物的脑细胞和/或神经细胞，预防或治疗疾病，保护脂肪酸酰胺水解酶的活性并预防或治疗哺乳动物的脑部睡眠障碍-神经保护和治疗或预防剂，试剂盒和生产化合物的方法

5. viral, cloning or expression vector, transgenic microorganism, pharmaceutical composition, kit for detecting an htrt polynucleotide or fragment thereof, uses of a polynucleotide, a peptide, a transgenic microorganism and a vector, and methods for obtaining a htrt recombinant peptide, to inhibit htrt expression, to increase the replicative ability of a cell, to detect an htrt polynucleotide or fragment thereof, to evaluate a condition associated with altered htrt expression, to express a heterologous gene, to selectively extinguish a trt-expressing cell, and to map a test compound for an ability to modulate trt promoter activity [P] . BRPI9712254B1 . 2019-01-15

机译：病毒，克隆或表达载体，转基因微生物，药物组合物，用于检测htrt多核苷酸或其片段的试剂盒，多核苷酸，肽，转基因微生物和载体的用途以及获得htrt重组肽以抑制htrt的方法表达，以增加细胞的复制能力，检测htrt多核苷酸或其片段，评估与htrt表达改变有关的条件，表达异源基因，选择性灭绝表达trt的细胞，并进行检测具有调节trt启动子活性的能力的化合物

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10. Poster session 2Morphogenetic mechanisms290MiR-133 regulates retinoic acid pathway during early cardiac chamber specification291Bmp2 regulates atrial differentiation through miR-130 during early heart looping formationDevelopmental genetics294Association of deletion allele of insertion/deletion polymorphism in alpha 2B adrenoceptor gene and hypertension with or without type 2 diabetes mellitus295Association of G1359A polymorphism of the endocannabinoid type 1 receptor (CNR1) with coronary artery disease (CAD) with type 2 diabetes mellitusCell growth, differentiation and stem cells - Vascular298Gamma-secretase inhibitor prevents proliferation and migration of ductus arteriosus smooth muscle cells: a role of Notch signaling in postnatal closure of ductus arteriosus299Mesenchymal stromal-like cells (MLCs) derived from induced pluripotent stem (iPS) cells: a promising therapeutic option to promote neovascularization300Sonic Hedgehog promotes mesenchymal stem cell differentiation to vascular smooth muscle cells in cardiovacsular disease301Proinflammatory cytokine secretion and epigenetic modification in endothelial cells treated LPS-GinfivalisCell death and apoptosis - 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