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SYSTEMS, DEVICES AND METHODS FOR SEQUENTIAL ANALYSIS OF COMPLEX MATRIX SAMPLES FOR HIGH CONFIDENCE BACTERIAL DETECTION AND DRUG SUSCEPTIBILITY PREDICTION USING A FLOW CYTOMETER
SYSTEMS, DEVICES AND METHODS FOR SEQUENTIAL ANALYSIS OF COMPLEX MATRIX SAMPLES FOR HIGH CONFIDENCE BACTERIAL DETECTION AND DRUG SUSCEPTIBILITY PREDICTION USING A FLOW CYTOMETER
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机译:用于高浓度细菌检测和使用流式细胞仪预测药敏性的复杂基质样品的序列分析系统,装置和方法
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摘要
Method for testing a sample of body fluid for the presence of bacteria and comprising the steps of adjusting the dilution of the sample to a predetermined concentration, of dividing the diluted sample into two batches defining a baseline-batch and a control-batch, of testing the baseline-batch at a time T0 with the flow cytometer to obtain enumerative baseline bacterial values, of culturing the control-batch in growth media between times T0 and T1, of testing the control-batch at time T1 with the flow cytometer to obtain enumerative control bacterial values, and of comparing the control values to the baseline values to determine a bacteria growth-ratio.
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