METHOD AND SYSTEM FOR THE DETECTION OF ACIDIC GLIAL FIBER PROTEIN (GFAP), IN PARTICULAR IN NEWBORNS AND PREMATURE INFANTS

摘要

The present invention relates to a method for the detection of acid glial fiber protein (GFAP) in the blood, especially in the blood of newborn or premature babies by PCR, which is also applicable for detection in the blood of premature and premature babies immediately after birth and so quickly and reliably feasible that an umbilical cord blood therapy decision can be made in premature and premature babies before more severe brain damage occurs. Techniques known in the art for the determination of GFAP in the blood of a mammal are not applicable to premature and premature infants because too much blood is needed. The method according to the invention provides the precondition for a therapeutic use of umbilical cord blood stem cells for the prevention and therapy of early childhood brain damage, which may develop into infantile cerebral palsy. There is no other possibility. According to the invention, a method for the detection of acid glial fiber protein (GFAP) in the blood of a mammal is provided in which GFAP is determined by means of PCR-amplified immunoassay (PCR-amplified immunoassay, I-PCR). The method according to the invention for the detection of acid glial fiber protein (GFAP) in the blood of a mammal by means of I-PCR is preferably combined with other methods to form a system which immediately increases the accuracy in detecting brain damage caused by oxygen deficiency, especially in newborns and premature babies Birth, allowed. These are the determination of the head circumference and the determination of the NO partial pressure in the respiratory gas.