A DIAGNOSTIC STRIP FOR DETERMINING THE AMOUNT OF SARCOSINE, CREATININE AND HYDROGEN PEROXIDE IN A BIOLOGICAL OR ENVIRONMENTAL SAMPLE

摘要

The present invention relates to a diagnostic strip for an enzymatic test for determining the amount of sarcosine and creatinine based on the formed hydrogen peroxide in a biological sample or in an environmental sample with an option of a visual electronic evaluation by evaluating the intensity of the colour product of the reaction. Zones of an absorbent matrix are applied to the diagnostic strip, where the first reaction zone for the determination of sarcosine contains sarcosine oxidase, sodium salt of 3-(N-ethyl-3-methylaniline) propanesulfonic acid and phenol, and the second reaction zone contains peroxidase and 4-amino-2,3-dimethyl-1-phenyl-3-pyrazole, or the strip in the reaction zone may contain 3,3'-diaminobenzidine, o-Phenylenediamine or ammonium salt of 2.2'-azino-bis(3-ethylbenzothiazoline-6) sulfonic acid. The creatinine diagnostic strip contains in the first reaction zone creatinase, sarcosine oxidase, ascorbate oxidase, catalase, sodium salt of 3-(N-ethyl-3-methylaniline) propanesulfonic acid and phenol and the second reaction zone contains creatininase, peroxidase and 4-amino-2,3-dimethyl-1-phenyl-3-pyrazole or 3,3'-diaminobenzidine or o-Phenylenediamine. The diagnostic strip for determining the amount of hydrogen peroxide contains in the first reaction zone sodium salt of 3-(N-ethyl-3-methylaniline) of propanesulfonic acid or 3,3'-diaminobenzidine or o-Phenylenediamine or ammonium salt of 2.2'-azino-bis(3-ethylbenzothiazoline-6) sulfonic acid and in the second reaction zone contains peroxidase and 4-amino-2,3-dimethyl-l-phenyl-3-pyrazole.