LARGE-SCALE CELL CULTURE SYSTEM AND VESSEL-VESSEL CELL LIQUID TRANSFER DEVICE FOR USE THEREIN, AND ROTARY CELL CULTURE DEVICE

摘要

Provided is large-scale cell culture system which can perform, in the absence of feeder cells or a coating material, large-scale culture of pluripotent stem cells, in particular, iPS cells, to be used in regenerative medicine or the like, while maintaining undifferentiation property thereof, which can perform subculture while eliminating variation due to skills of operators, and which can perform culture of adhesive cells in a suspended state; an inter-vessel cell liquid transfer device to be used therein; and a rotary cell culture device. This large-scale cell culture system is for performing large-scale culture of cells by performing, in a closed system, subculture and transfer of spheroids and a culture medium by use of a vessel having a syringe structure, wherein the vessel comprises a front flange and a back flange which have a same circular outer shape and which are provided integrally with both ends of an outer cylinder part of the vessel, and the vessel allows rotary culture, utilizing the front flange and the back flange in a state where a head of the vessel is closed by a detachable cap and a space, in the vessel, closed by a gasket of a plunger is filled with a cell liquid obtained by suspending cells in a culture medium.