METHODS AND COMPOSITIONS FOR GENERATING CHONDROCYTE LINEAGE CELLS AND/OR CARTILAGE-LIKE TISSUE

摘要

To provide methods and compositions for generating chondrocyte lineage cells and/or cartilage-like tissue.SOLUTION: Disclosed herein is a method comprising the steps of: a. culturing a primitive streak-like mesoderm population with a culture medium comprising a FGF agonist and a BMP inhibitor etc. to specify a CD73+, CD105+ and/or PDGFR-β+ paraxial mesoderm population; b. generating a chondrocyte precursor population by culturing the paraxial mesoderm population at a high cell density in a serum free medium with a TGFβ agonist to produce a high cell density Sox9+, collagen 2+ chondrocyte precursor population; and c. either culturing the high cell density chondrocyte precursor population with TGFβ3 agonist for an extended period of time to produce an articular like non-hypertrophic chondrocyte cells and/or cartilage-like tissue or culturing the high cell density chondrocyte precursor population with a BMP4 agonist for an extended period of time to produce a hypertrophic chondrocyte like cells and/or cartilage-like tissue.SELECTED DRAWING: None