Metabolic labeling and molecular enhancement of biomaterials using bioorthogonal reactions

摘要

The present application relates to mammalian organs by administering a nutrient (eg, peracetylated N-azidogalactosamine Ac4GalNAz) to a mammal or culturing the organ or tissue in a bioreactor containing such nutrients. Alternatively, a method for functionalizing a tissue is provided. The application also provides a method of selectively functionalizing the extracellular matrix (ECM) of a mammalian organ or tissue by administering a nutrient (eg, peracetylated N-azidogalactosamine Ac4GalNAz) to the mammal. To do. In some aspects, the application is a decellularized scaffold of a mammalian organ or tissue comprising an extracellular matrix, wherein the extracellular matrix of the decellularized scaffold is a chemical group that is reactive in a bioorthogonal chemical reaction. Provides a decellularized scaffold, eg functionalized with an azide chemical group. The present application also provides biological prosthetic meshes prepared according to the methods of the present application and mammalian organs and tissues for implantation.