The present invention relates to an image analysis method for analyzing IHC tissue samples (100). The method stores the set ID in the spatial database (318) of the spatial DBMS (316) for each of one or more sets (112; 113) of the digital images (106.1 to 106-4; 106.5 to 106.7) (702), wherein the digital images of each digital image set show the same layer (102) or adjacent layers (102; 110; 108) of the IHC tissue sample, step (702); Storing, for each, a plurality of spatial data objects in the spatial database in association with the set ID (704), each spatial data object being a point, a line, a polygon, or a combination thereof, and Creating at least one spatial index covering spatial data objects of the digital image contained in said one or more sets, step (704) representing the biomedical features; Selecting (706) one of the one or more sets of digital images; and spatial data of two or more digital images of the selected set of digital images by the spatial DBMS. Applying a spatial database operation to the at least one spatial index to determine the relative position of the object (710); the relative position is represented by a spatial data object of the two or more digital images Providing (712) as an input to determine the distance and / or relative position of the biomedical features of the tissue sample.
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