System and method for time-resolved fluorescence immunoassay detection

摘要

A system (100) for time-resolved fluorescence immunoassay detection is disclosed. The system (100) includes a container (110) with a sample volume adapted to receive a sample (99) therein, a light source (120) adapted to emit pulsed excitation light (200), Illumination optics (130) adapted to collect the pulsed excitation light (200) from the light source (120) and deliver the pulsed excitation light (200) to the sample volume in the container (110), and at least detection A detection device (140) adapted to gate detect fluorescent radiation in the spectral range, and at least within the detection spectral range, the fluorescent radiation (210) is collected from the container (110) and applied to the detection device (140); Detection optics (150) adapted to deliver fluorescent radiation (210) and an optical filter configured to separate excitation light (200) and detection light (210) It includes unit (160), the. The light source (120) is a light emitting diode having peak intensity radiation at a wavelength of less than 355 nm. The system (100) further includes a control unit (101) configured to at least control the timing of the detection cycle. The detection cycle includes an excitation pulse emitted by the light emitting diode (120), followed by a detection period for detection of fluorescent emission (210) by the detection device (140), the detection period being due to a detection delay. Separated from the excitation pulse. The pulse duration of the excitation pulse is at least 10 μs. According to a further aspect, a method for time-resolved fluorescence immunoassay detection is provided.