Self-organized patterning using patterned hydrophobic surfaces

摘要

Embodiments provided herein are directed to self-assembled methods of preparing patterned surfaces for sequencing applications including, for example, patterned flow cells for digital fluidic devices or patterned surfaces It is said. The method utilizes photolithography to create a surface patterned with multiple microscale or nanoscale contours separated by hydrophobic interstitial regions and does not require oxygen plasma treatment during the photolithography process. Furthermore, the method avoids the use of any chemical or mechanical polishing step after depositing gel material on the contour.