METHODS AND KITS FOR DETECTING AZOLE-RESISTANT ASPERGILLUS FUMIGATUS

摘要

To provide simple methods for detecting Aspergillus fumigatus resistant to azole agents.SOLUTION: Disclosed herein is a method for determining the repetition number in a repetitive base sequence, the method comprising a first step of hybridizing fluorescently labeled probes having FRET effect with a base sequence in the repetitive base sequence and with a base sequence outside the repetitive sequence respectively, a second step of amplifying a region comprising the repetitive sequence using PCR, a third step of determining the fluorescent intensity generated from the fluorescently labeled probes degraded during the PCR reaction and a fourth step of comparing the fluorescent intensity of the fluorescently labeled probe hybridized with the repetitive base sequence and the fluorescent intensity of the fluorescently labeled probe hybridized with the base sequence outside the repetitive base sequence.SELECTED DRAWING: Figure 1