Method inducing cardiomyocytes are from multipotential stem cell, the preferred method of culture medium additive, difference induction voltage regulator, the kit of culture medium, for making culture medium, and for inducing cardiomyocytes from pluripotent stem cell

摘要

It solves the problems, such as: a kind of induction differentiation cardiac muscle cell being provided can manufacture the cardiac muscle cell of high quality from multipotential stem cell and largely use, stablize, it is cheap simple, in the preferred method of culture medium additive, difference induction voltage regulator, culture medium, for making culture medium, for inducing differentiation cardiac muscle cell from multipotential stem cell. ;SOLUTION: including: that embryoid forms the multipotential stem cell for the culture that suspends and forms embryoid in a kind of slave multipotential stem cell of inducing cardiomyocytes; A kind of embryoid induction mesoderm of mesoderm Induction Process suspension culture; In cardiac muscle cell's Induction Process in such a way the embryoid induction cardiac muscle cell of mesoderm induction suspension culture, it includes first cardiac muscle cell's induction processing that the first cardiac muscle cell induces processing that cardiac muscle cell, which incudes process, and culture medium is a specific culture medium in each process. ;In selected attached drawing: none;COPYRIGHT:(C) 2016, JPO& INPIT