System and method for segmentation of 3D microscopic images

摘要

A system for segmenting an image captured from an image capture device of a high content imaging system includes an image acquisition module that receives the image captured by the image capture device. The coarse object detection module develops the coarse segmented image, and each pixel of the coarse segmented image is identified as one of the object and the background pixel. The marker identification module selects a marker pixel from the pixels of the coarsely segmented image, each marker pixel being the farthest from the background pixel relative to the neighboring pixels of the group, among the group of adjacent object pixels in the coarsely segmented image. It is one of. The object segmentation module associates each object pixel in the coarsely segmented image with a marker pixel, and the distance-based metric between the object pixel and the marker pixel is determined from the object pixel and any other marker pixel in the coarsely segmented image. Is less than the distance-based metric.