Thermal stability improves, and the 1 of lysine decarboxylase enzyme variant,The preparation method of 5- pentanediamines, wherein use

摘要

The present invention provides methods and means of producing 1,5-PD efficiently in a manner suitable for an actual production. Specifically, the present invention provides a method of producing 1,5-pentamethylenediamine comprising forming 1,5-pentamethylenediamine from L-lysine and/or a salt thereof by an action of a mutated lysine decarboxylase having mutation(s) of one or more amino acid residues that improve a thermal stability in any amino acid sequence of (a) the amino acid sequence of SEQ ID NO:1, (b) an amino acid sequence comprising one or several amino acid residue substitutions, deletions, insertions or additions in the amino acid sequence of SEQ ID NO:1, or (c) an amino acid sequence having 90% or more identity to the amino acid sequence of SEQ ID NO:1, and having a lysine decarboxylation activity; and the like.