For by biomolecule fixation in the functionalized nucleosides-shell of two sulphur-dithiocarbamate and carrier on the surface of solids

摘要

This invention is related to nucleic acid chemistry and describes novel 1,2-dithiolane functionalized nucleoside phosphoramidites (1, Chart 1) and corresponding solid supports (2, Chart 1). In addition to these derivatives, 1,2-dithiolane moiety can also be functionalized to at the various positions of the nucleobase and sugar part as shown in Schemes 1 to 8. The nucleosides of our invention carry a primary hydroxyl for DMTr (4,4′-dimethoxytrityl) function for chain elongation. Furthermore, the phosphoramidite function is attached at the 3′-hydroxyl of the nucleoside. This allows oligonucleotide chain extension under standard DNA and RNA synthesis chemistry conditions and techniques, thus leading to high quality oligonucleotides. These derivatives are useful for introduction of reactive thiol groups either at 3′- or 5′-end of the oligonucleotides on the solid supports such as gold, silver and quantum dots.