Differentiation induction from human pluripotent stem cells to hypothalamic neurons

摘要

Provided is a method for efficiently inducing differentiation from human pluripotent stem cells into hypothalamic neurons. Also provided is a method for constructing a cell structure in which hypothalamic tissue and pituitary tissue are integrated from human pluripotent stem cells. Suspension culture of aggregates of human pluripotent stem cells in a medium containing a low concentration of an osteogenic factor signaling pathway activator and a low concentration of an Shh signaling pathway activator, and cell aggregation obtained in this step A cell structure containing hypothalamic tissue is obtained by a method comprising the step of further suspension culture of the mass in a medium containing a low-concentration Shh signal pathway agent. In addition, a suspension culture of aggregates of human pluripotent stem cells in a medium containing a bone morphogenetic factor signal transduction pathway activator and a Shh signal pathway agonist, and the cell aggregates formed in this step Further suspension culture in a medium containing a substance that activates the formation factor signal transduction pathway and an agent that acts on the Shh signal pathway, and the cell aggregate obtained in this step in a medium suitable for simultaneous induction of the pituitary and hypothalamus A cell structure including a hypothalamic tissue and a pituitary tissue is obtained by a method including the step of suspension culture in (1).