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System and method for analyzing splicing codes of spliceosomal introns
System and method for analyzing splicing codes of spliceosomal introns
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机译:分析剪接内含子剪接编码的系统和方法
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摘要
A system and method for analyzing splicing codes of spliceosomal introns is disclosed. One embodiment comprises methods of identifying introns and exons in genomic DNA or pre-mRNA sequences by locating characteristic markers in splicing junctions by computation and/or manually. Exon sequences predicted by computation can be verified and characterized by employing standard amplification methods, such as comparative genomic, RNA-seq, next-generation sequencing, RT-PCR. DNA/RNA/oligo, electrophoretic or protein chip technologies. If a given sample is verified, its polypeptide can be translated based on genetic codons. Its functions can be deduced based on its characteristics, computation predictions and related knowledge databases. These data can be used to compare databases which correlate the characterized intron or exon or gene to characterized diseases or genetic mutations. Isoforms can be detected and analyzed at mRNA and protein levels alone and with other isoforms predicted by computation, characterized by experiments and stored in existing databases.
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机译:公开了一种用于分析剪接内含子的剪接码的系统和方法。一个实施方案包括通过通过计算和/或手动将特征标记定位在剪接连接处来鉴定基因组DNA或前mRNA序列中内含子和外显子的方法。通过计算预测的外显子序列可以通过采用标准的扩增方法进行验证和表征,例如比较基因组,RNA-seq,下一代测序,RT-PCR。 DNA / RNA /寡核苷酸,电泳或蛋白质芯片技术。如果验证了给定的样品,则可以基于遗传密码子翻译其多肽。可以根据其特征,计算预测和相关的知识数据库来推导其功能。这些数据可用于比较将特征内含子或外显子或基因与特征性疾病或遗传突变相关的数据库。可以分别在mRNA和蛋白质水平以及通过计算预测的其他同工型检测和分析同工型,以实验为特征并存储在现有数据库中。
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