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Methods and compositions for improving removal of ribosomal RNA from biological samples
Methods and compositions for improving removal of ribosomal RNA from biological samples
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机译:改善从生物样品中去除核糖体RNA的方法和组合物
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摘要
The invention generally relates to compositions for maximizing capture of affinity-labeled molecules on solid supports. The disclosed methods and compositions were developed to maximize depletion of ribosomal RNA from total RNA samples, which is useful to improve the quality of RNA preparations used for applications such as massively parallel sequencing. The RNA depletion method is based on using long affinity-labeled RNA molecules that are complementary to all or part of the target ribosomal RNAs, as subtractive hybridization probes.
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