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SINGLE EXTRACELLULAR VESICLE MULTIPLEXED PROTEIN AND RNA ANALYSIS
SINGLE EXTRACELLULAR VESICLE MULTIPLEXED PROTEIN AND RNA ANALYSIS
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机译:单细胞外囊复合蛋白和RNA分析
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摘要
A system for identifying exosome subpopulations and payloads for potential targeted therapeutics comprises a simultaneous extracellular vesicle membrane and enclosed content detecting composition. The extracellular vesicle membrane and enclosed content detecting composition includes: a bead comprising a selective binding agent; an extracellular vesicle membrane protein-specific DNA tag bound to the selective binding agent and comprising a poly-T end; and an extracellular vesicle-derived RNA comprising a poly-A end bound to the membrane protein-specific DNA tag poly-T end. The composition may comprise cDNA bound to the RNA. The selective binding agent may comprise a barcode and a molecular identifier. The selective binding agent may selectively bind the membrane protein identifier DNA present in a droplet. In some embodiments, the bead may be a magnetic bead. In some embodiments, the bead may be a conductive bead.
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