首页>
外国专利>
Cell culture substrate comprising a laminin and a cadherin
Cell culture substrate comprising a laminin and a cadherin
展开▼
机译:包含层粘连蛋白和钙黏着蛋白的细胞培养底物
展开▼
页面导航
摘要
著录项
相似文献
摘要
The present disclosure related to isolated laminin-521, methods for making recombinant laminin-521, host cells that express recombinant laminin-521, and compositions containing laminin-521. Laminin-521 can maintain stem cells in vitro pluripotency, enable self-renewal, and enable single cell survival of human embryonic stem cells. When pluripotent human embryonic stem cells are cultured on plates coated with recombinant laminin-521 (laminin-11), in the absence of differentiation inhibitors or feeder cells, the embryonic stem cells proliferate and maintain their pluripotency. It has also been discovered that human recombinant laminin-521 (laminin-11) provides single cell survival of stem cells after complete dissociation into a single cell suspension. Useful cell culture mediums containing at most 3.9 ng/ml of beta fibroblast growth factor (bFGF) are also described herein.
展开▼
机译:本公开涉及分离的层粘连蛋白521,制备重组层粘连蛋白521的方法,表达重组层粘连蛋白521的宿主细胞和包含层粘连蛋白521的组合物。 Laminin-521可以在体外维持干细胞的多能性,实现自我更新,并使人类胚胎干细胞单细胞存活。当多能人胚胎干细胞在涂有重组层粘连蛋白-521(laminin-11)的平板上培养时,在没有分化抑制剂或饲养细胞的情况下,胚胎干细胞会增殖并保持其多能性。还已经发现,人重组层粘连蛋白521(层粘连蛋白11)在完全分解成单个细胞悬液后提供干细胞的单个细胞存活。本文还描述了包含至多3.9 ng / mlβ成纤维细胞生长因子(bFGF)的有用细胞培养基。
展开▼