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Method for producing L-citrulline by using a recombinant Corynebacterium crenatum strain

机译：利用重组 Crenatum 菌株生产L-瓜氨酸的方法

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摘要

Disclosed is a method for producing L-citrulline using recombinant Corynebacterium crenatum cells as whole-cell biocatalysts. The present invention provides a recombinant C. crenatum that expresses an exogenous arginine deiminase gene from Lactobacillus brevis. The recombinant C. crenatum SDNN403 is used as biocatalysts for converting L-arginine to produce L-citrulline. Using the method of the invention, the concentration of L-citrulline reached 301.4 g/L after a 48 hr conversion reaction, and the molar conversion rate reached 99.9%.

机译：公开了一种使用重组肾上腺棒状杆菌细胞作为全细胞生物催化剂生产L-瓜氨酸的方法。本发明提供了重组IC。表达来自短乳杆菌的外源性精氨酸脱亚氨酶基因的云杉。重组。 crenatum SDNN403用作将L-精氨酸转化为L-瓜氨酸的生物催化剂。使用本发明的方法，在48小时的转化反应后，L-瓜氨酸的浓度达到301.4g / L，并且摩尔转化率达到99.9％。 展开▼

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公开/公告号US10125383B2

专利类型

公开/公告日2018-11-13

原文格式PDF

申请/专利权人 ZHIMING RAO;MEIZHOU WANG;MEIJUAN XU;XIAN ZHANG;TAOWEI YANG;
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申请/专利号US201815876478

发明设计人 ZHIMING RAO;MEIZHOU WANG;MEIJUAN XU;XIAN ZHANG;TAOWEI YANG;
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申请日2018-01-22

分类号C12P13/10;C12N15/77;C12N9/78;C12R1/15;

国家 US

入库时间 2022-08-21 12:12:14

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