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TANDEM FOLDING METHODS TO IMPROVE PROTEIN FOLDING YIELD
TANDEM FOLDING METHODS TO IMPROVE PROTEIN FOLDING YIELD
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机译:串联折叠法提高蛋白质折叠率
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摘要
Methods of refolding proteins are provided, especially cysteine-containing proteins such as insulin, proinsulin, and analogues thereof. The methods make used of tandem folding via the addition, at two different time, of two different reducing agents. A first reversible reducing agent can be added to induce folding and, at a later time, a second irreversible reducing agent can be added to prevent and/or reverse the formation of aggregates via intermolecular sulfide bonds. The methods can be used to reform a variety of cysteine-containing proteins with high yield of the native form, e.g. about 60 mol %, 70 mol %, or more.
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