首页> 外国专利> METHOD FOR PREPARING ADHERENT-TYPE CELLS ACCLIMATED TO SUSPENSION CULTURE, METHOD FOR INDUCING EPITHELIAL-MESENCHYMAL TRANSITION IN ADHERENT-TYPE EPITHELIAL CELLS, AND USE FOR METHODS

METHOD FOR PREPARING ADHERENT-TYPE CELLS ACCLIMATED TO SUSPENSION CULTURE, METHOD FOR INDUCING EPITHELIAL-MESENCHYMAL TRANSITION IN ADHERENT-TYPE EPITHELIAL CELLS, AND USE FOR METHODS

机译:悬浮培养的贴壁细胞的制备方法,贴壁上皮细胞的上皮-间质转化的诱导方法及其用途

摘要

The present invention is: a method for preparing adherent cells acclimated to suspension culture, including a step of culturing the adherent cells in contact with an alicyclic structure-containing polymer formed article; a method of producing a nucleic acid or a protein encoded by a foreign gene by culturing the adherent cells acclimated to suspension culture in accordance with the aforementioned method; a promotor for suspension culture acclimation of the adherent cells, composed of an alicyclic structure-containing polymer formed article; the use of an alicyclic structure-containing polymer formed article for acclimating an adherent cells to suspension culture in a liquid medium; a vessel for suspension culture acclimation of an adherent cells, having at least a bottom surface composed of an alicyclic structure-containing polymer formed article; a method for inducing epithelial-mesenchymal transition, including a step of culturing adherent epithelial cells in contact with an alicyclic structure-containing polymer formed article; and so on.
机译:本发明是:制备适于悬浮培养的贴壁细胞的方法,其包括与含有脂环式结构的聚合物成型体接触培养贴壁细胞的步骤。通过按照上述方法培养适应悬浮培养的贴壁细胞来生产由外源基因编码的核酸或蛋白质的方法;用于使贴壁细胞悬浮培养适应的促进剂,其由含有脂环结构的聚合物成型体构成;含脂环结构的聚合物成型制品用于使粘附细胞适应在液体培养基中悬浮培养的用途;用于使贴壁细胞悬浮培养适应的容器,其至少底面由含有脂环式结构的聚合物成型体构成;一种诱导上皮-间质转化的方法,包括培养与含有脂环式结构的聚合物成型体接触的粘附上皮细胞的步骤。等等。

著录项

  • 公开/公告号US2018355321A1

    专利类型

  • 公开/公告日2018-12-13

    原文格式PDF

  • 申请/专利权人 ZEON CORPORATION;

    申请/专利号US201615781250

  • 发明设计人 TAKAAKI HIRANO;YUMIKO MIMURA;

    申请日2016-12-16

  • 分类号C12N5/0775;

  • 国家 US

  • 入库时间 2022-08-21 12:11:55

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