MICRO-FLUIDIC CHIP AND ITS MODIFICATION METHOD AND APPLICATION IN DETECTION OF THE QUANTITY OF FOOD BACTERIA

摘要

The present invention discloses a micro-fluidic chip and its modification method and application in detection of the quantity of food bacteria, comprising the following steps: changing functional group —CH₃ on the internal surface of micro-fluidic channel of micro-fluidic chip to functional group —OH through modification; Supplying amino silane reagent to the micro-fluidic channel; supplying dendritic polymer as modified by —COOH to internal surface of the micro-fluidic channel after drying; grafting primer of aminated aptamer RCA on terminal 5′ and hybrid from its padlock probe on dendrimer on internal surface of the micro-fluidic channel; wherein, the padlock probe is the complementary sequence of the aptamer of the target pathogenic bacteria to be tested; after that, supplying RCA reaction reagent to the micro-fluidic channel to make aptamer RCA generate long-chain aptamer in series. The present invention adopts two RCA reactions of varied functions in combination, and uses dendritic polymer to modify internal surface of the chip so as to improve identification and snatch of targets and signal enhancement, and realize integrated detection.