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Peptide tag system that spontaneously form an irreversible link to protein partners via isopeptide bonds
Peptide tag system that spontaneously form an irreversible link to protein partners via isopeptide bonds
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机译:肽标签系统通过异肽键自发形成与蛋白质伴侣的不可逆连接
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摘要
Described herein is-a protein capable of spontaneously forming an isopeptide bond for the development of a peptide tag and binding partner pair wherein the peptide tag and binding partner are capable of covalently binding to each other via an isopeptide bond. Also provided is a method for developing a peptide tag and binding partner pair which are capable of covalently binding to each other based on a protein which is capable of spontaneously forming an isopeptide bond. Additionally provided are peptide tag and binding partner pairs which are obtainable from isopeptide proteins. Further, specifically developed peptide tags and binding partners are described, together with nucleic acid molecules and vectors which encode those peptides or proteins.
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