METHODS FOR EXTRACTING AND MEASURING CONCENTRATIONS OF BIOMOLECULES IN COMPLEX MATRICES WITHOUT THE NEED FOR IMMUNOCAPTURE

摘要

Extraction and separation methods that can be used to quantify the absolute concentrations of one or more low abundance biomolecules present in biological fluids and tissues, without the need for antibody enrichment or reliance on an antibody for quantification are provided. These biomolecules can be biomarkers used to diagnose and monitor disease progression. As an example, these methods are applied to human plasma and CSF, and used to quantify Alzheimer's disease biomarkers.