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Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
Temperature shift for high yield expression of polypeptides in yeast and other transformed cells
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机译:温度变化可在酵母和其他转化细胞中高产率表达多肽
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摘要
Methods for producing heterologous proteins are disclosed. In particular, the present disclosure provides improved methods of producing desired proteins, including multi-subunit proteins such as antibodies, with a higher yield and improved purity. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris. 展开▼