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High-Resolution, Real-Time Imaging With Adaptive Optics and Lattice Light Sheets
High-Resolution, Real-Time Imaging With Adaptive Optics and Lattice Light Sheets
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机译:自适应光学元件和点阵光片的高分辨率实时成像
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摘要
A microscope directs light through an excitation objective to generate a lattice light sheet (LLS) within a sample. A detection objective collects signal light from the sample in response to the LLS and images the collected light onto a detector. Second and third light beams are imaged onto focal planes of the excitation objective and detection objective, respectively. One or more wavefront detectors determine wavefronts of light emitted from the sample and through the excitation objective in response to the imaged second light beam and emitted from the sample through the detection objective in response to the imaged third light beam. A wavefront of the first light beam is modified to reduce a sample-induced aberration of the LLS within the sample, and a wavefront of the signal light emitted from the sample is modified to reduce a sample-induced aberration of the signal light at the detector.
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