ENHANCED ORGANOID FORMATION AND INTESTINAL STEM CELL RENEWAL

摘要

High mobility group A1 (Hmga1) chromatin remodeling proteins are enriched in intestinal stem cells (ISCs), although their function in this setting was unknown. The present invention describes a method of producing a genetically modified organoid comprising the following steps: isolating a crypt of the small intestine; culturing a portion of the crypt in a culture medium; forming an organoid; and transducing the organoid with a vector comprising Hmga1. Methods using genetically modified organoids to treat or prevent injuries including cancer are described.