METHODS AND KITS FOR ANALYSING THE STEADY-STATE ACTIVATION OR INHIBITION OF ITAM SIGNALLING OF IMMUNORECEPTORS IN BLOOD LEUKOCYTES

摘要

The present invention relates to methods and kits for analysing the steady-state activation or inhibition of ITAM signalling of immunoreceptors in blood leukocytes. The inventors investigated that low valency ligands induced ITAMi signals by FcR, but also BCR and TCR, that were driven by Lyn or Lck recruitment followed by SHP-1 activation resulting in inhibition of heterologous activating receptors. In contrast, Fyn was required for multivalent ligand induced ITAM signals driving Syk or Zap-70 recruitment resulting in cell activating functions. In particular, the present invention relates to a method for analysing the activating or inhibiting steady-state of ITAM signalling of immunoreceptors in a population of leukocytes comprising by determining whether the immunoreceptors are associated with a Fyn-kinase activity or are associated with a Lyn/Lck kinase activity.