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Nanopore Discrimination of Target Polynucleotides from Sample Background by Fragmentation and Payload Binding
Nanopore Discrimination of Target Polynucleotides from Sample Background by Fragmentation and Payload Binding
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机译:通过片段化和有效载荷结合从样品背景中对目标多核苷酸进行纳米孔识别
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摘要
Disclosed herein are methods and compositions for detecting a target DNA sequence from a sample that does not require sample purification or amplification. The method uses fragmentation, sequence-specific binding or ligation of probes, and payload molecules for selective detection of the target-sequence using a nanopore sensor.
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