METHODS AND COMPOSITIONS FOR TRANSPOSITION USING MINIMAL SEGMENTS OF THE EUKARYOTIC TRANSFORMATION VECTOR PIGGYBAC

摘要

Isolated nucleic acid molecules that include a minimally-sized, functional (minimally-functional) piggyBac transposon can incorporate (i) a 5′ internal domain (ID) comprising a nucleotide fragment that is substantially homologous to a native piggyBac transposon sequence; (ii) a 5′ terminal repeat domain (TRD) comprising a 5′ terminal repeat (TR) sequence, a 5′ spacer sequence, and a 5′ internal repeat (IR) sequence; (ii) a sequence of interest; (iv) a 3′ TRD comprising a 3′ IR sequence, a 3′ spacer sequence, and a 3′ TR sequence; and (iv) a 3′ ID comprising a nucleotide fragment that is substantially homologous to the native piggyBac transposon sequence. The 5′ TRD and the 3′ TRD can be optionally linked by a sequence comprising a multiple cloning site.