Protein Quantification by Near Infrared Spectral Imaging

摘要

The present invention introduces a protein/peptide quantification method based on near infrared spectral imaging of protein gel bands obtained from gel electrophoresis methodologies. Spectral imaging is a fast and reliable method that is simple to use and easily applicable to several procedures. When proteins are separated through gel electrophoresis, the gel is imaged using a near infrared spectrometer and a standard curve is used to calculate the protein/peptide content based on its relative near infrared absorbance to standard proteins. Compared to other protein quantification techniques, the methods of the present invention quantifies the proteins separated by gel electrophoresis without the need for contrast reagents or for purifying protein out of the gel, shortening the processing time, reducing the number of steps involved, and eliminating the need for additional chemicals.