A method for measuring non-single components, including non-single components and polymers

摘要

1. A method for quantifying nonionic surfactant components, including nonionic surfactant components and polypeptide components, including (a) steps for applying components to mixed ion or ion exchange chromatography materials;Input of chromatographic substances into a solution consisting of mobile phase A and mobile phase B.A. Moving stage A contains ammonium hydroxide or hydrogen acid, while moving stage B contains ammonium hydroxide in organic solvent or acid in methanol, respectively.(b) Removal of polypeptides from ionic or ion exchange chromatographic materials in a mixed manner and adoption of a solution including mobile A and B phases,A. The ratio of B-class to A-class is higher than that of (a) class.(c) Avoiding non-ionic tension,O Non-ionic surfactants and polypeptides separated from chromatographic materials include mobile phase A and mobile phase B. In these two phases, the ratio of mobile phase B to mobile phase A is higher than that of mobile phase C.(d) Quantification of non-ionic surface activity;The interference of nonionic surfactants and polypeptides was reduced in the quantitative process.