Recombinant worm vaccine produced by Pichia Pastoris and protein production and purification process as worm vaccine

摘要

The current situation involves the production of recombinant proteins with high protein expression of synthetic genes in Pichia Pastoris. Specifically, the report describes the recombinant production of SM14 protein from Mansoni spongy cells, which creates a synthetic gene for the high concentration of this protein and clones it under the control of two herbage developers: sensible developers. Or methanol (AX1) and constituent salesman (GAP). In these buildings, Pichia Pastoris strains are genetically manipulated for efficient production.Antigen vaccine SM14. The process of producing and purifying this protein from P. Pastoris cells has also been improved and can be used for industrial scale production.