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High performance purification of Neisseria meningitis serum concentrate
High performance purification of Neisseria meningitis serum concentrate
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机译:高效脑膜炎奈瑟菌血清浓缩液的纯化
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摘要
1. Claim 1: a process for rapid purification of high-performance polyurethane from Neisseria meningidis serogax capsule; The process includes the following steps: (a) treating a serogroup x Neisseria meningitis fermentation medium by adding a predetermined simple salt solution and a specific concentration of dry salt powder; (b) adding a specific amount of organic solvent (a) to the salt treated solution;(c) mix (b) solution for a specific period of time at ambient temperature; (d) centrifuge (c) to RPM within a predetermined period of time; (E) collect excess water from (d) centrifugal solution;(f) the above-mentioned (E) filter is concentrated and filtered with molecular weight cutting membrane to obtain partially purified bacterial polytetrafluoroethylene; (g) under specified conditions, the partially purified bacterial polytetrafluoroethylene (f) is precipitated to (f) level together with at least one ionic detergent; 2. Specific RPM centrifugation is then carried out for a predetermined period of time to obtain the first pellet; (H) the first pellet is dissolved into an inorganic precipitant; (I) at least one organic solvent is added to the first dissolved pellet, and then incubation is carried out under predetermined conditions;(J) centrifuging the first stage solution under specific conditions, and then collecting the second pellet; (k) dissolving the second pellet into MQW, processing specific chemical reagents and specific organic solvents, and then incubating under specific conditions; (L) centrifugation of this (k) - grade latent solution under specific centrifugation conditions and collection of the filter; (m) carbon filtration of this (L) - grade filter in order to achieve the desired optical density before carbon filtration;(n) use the PES filtration arrangement, and then use the PES filtration and filtration arrangement under specific conditions to filter the (m) level carbon filter; (o) use the PES filtration arrangement to filter the filter to obtain purified polyurethane; (P) storage of this purified grade (o) polymer under specific conditions; in this process, the process is cost-effective, measurable and provides purified Neisseria meningitis type X polymer in the range of 147-323 mg / L fermentation medium.
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