methods for generating antigen-loaded dendritic cells and for eliciting an immune response in a mammal; and, preservative composition.

摘要

methods for generating antigen-loaded dendritic cells and for eliciting an immune response in a mammal; and, preservative composition. The present invention provides compositions and methods for generating and cryopreserving dendritic cells with superior functionality in producing stronger t-cell signals, resulting in a more potent dc-based antitumor vaccine. The present invention includes mature, antigen-loaded toll-equivalent receptor-activated antigen-activated dcs that induce clinically effective immune responses, preferably when used more in the early course of the disease process. The DNAs of the present invention produce desirable levels of cytokines and chemokines, yet have the ability to induce apoptosis of tumor cells. The cells may be cryopreserved and thawed for later use, thereby reducing the need for repeated apheresis and elutriation processes during vaccine production. These methods can also be used to directly target target molecules involved in carcinogenic signaling pathways and cancer stem cells.