lipid bilayers supported on porous nanoparticles (protocells) for targeted administration, including transdermal delivery of a charge and associated methods

摘要

"porous nanoparticle supported lipid bilayers (protocells) for targeted administration, including transdermal delivery of a charge and associated methods", as the present invention is protocells for specific targeting of hepatocellular cells and other cancer cells, which comprise a nanoporous silica core with a supported lipid bilayer; at least one cancer cell killing agent (such as a traditional small molecule, a macromolecular charge (e.g., siarn or a protein toxin, such as, for example, a-chain ricin toxin or a-chain diphtheria toxin) and / or a DNA plasmid packaged with a histone disposed within the nanoporous silica core (preferably supercoiled to more efficiently package the DNA in the protocells), which is optionally modified by a nuclear localization sequence, to aid in the localization of protocells within the cancer cell nucleus and the ability to express peptides involved in cancer cell therapy (apoptosis / cell death) or as a reporter, a targeting peptide that targets cancer cells in the tissue to be treated so that the binding of the protocell to the target cells is specific and enhanced and a fusogenic peptide that promotes the endosomal escape from protocells and encapsulated DNA. protocells according to the present invention may be used to treat cancer, including especially hepatocellular cancer (liver cancer), by the use of novel binding peptides (c-met peptides), which bind to selectively to hepatocellular tissue, or to function in the diagnosis of cancer, including cancer treatment and drug discovery. 1/1