VECTOR OF EXPRESSION ACTIVATED BY HEAVY METALS, METHOD FOR EXPRESSION AND PURIFICATION OF RECOMBINANT PROTEIN OF HIGH CHEMICAL COMPLEXITY, AND USES

摘要

"Heavy Metal Activated Expression Vector, Method for Expression and Purification of High Chemical Complexity Recombinant Protein, and Uses" The present invention is directed to a vector with heavy metal salt activated gene expression system and a method for the expression and purification of recombinant protein of high chemical complexity. such an expression vector has part of a heavy metal responsive promoter (mtlla), an extracellular secretion signal (signal peptide), an affinity purification motif (histidine tail), and a processing signal (cleavage site). tev), ensuring high expression in culture, a reduction in purification steps, and a high purification rate of the protein of interest. Such a vector can be used for the expression of non-limiting proteins for therapeutic use, food, enzymes for industrial fermentation processes.