VECTOR FOR EXPRESSING FLUORESCENT PROTEINS IN HAPLOID CELLS, EXPRESSION CASSETTES AND METHOD OF OBTAINING HAPLOIDES FROM SACCHAROMYCES CEREVISIAE ON LARGE SCALE

摘要

A vector for expressing fluorescent proteins in haploid cells, expression cassettes, and the large-scale saccharomyces cerevisiae haploid obtaining method The present invention relates to a vector, herein named pmf_002, and the method of separating and collecting haploid from s. cerevisiae, using said vector to express fluorescent proteins (markers) in haploid cells (kills and kills) and thus distinguish them from each other and between tetrads and diploids using flow cytometer. for such, said vector comprises the reporters egfp (enhanced green fluorescent protein) and cyofp1 (cyan orange fluorescent protein), the ste2 (sterile2) and ste3 (sterile3) promoters, the cyc1 (cytochrome c1) and idp1 (isocitrate dehydrogenase) terminators. 1), and the backbone vector (pgs_004).