rodent, isolated rodent cell or tissue, immortalized cell, rodent embryonic stem cell, rodent embryo, and methods for producing a rodent and an antibody

摘要

non-human animals are provided and methods and compositions for the production and use thereof, wherein said non-human animals have a genome comprising a manipulated or recombinant diversity cluster within an immunoglobulin heavy chain variable region, wherein the engineered or recombinant diversity cluster comprises an insertion of one or more coding sequences of a non-immunoglobulin polypeptide of interest. non-human animals described herein express antibodies characterized by complementarity determining regions (cdrs), in particular cdr3s with diversity, which direct binding to specific antigens. Methods for producing antibodies from non-human animals are also provided, wherein the antibodies contain human variable regions and mouse constant regions.