Method for Purifying Antifungal and Exopolysaccharide Compounds from a Microbial Cell Culture

摘要

The present invention provides a method of enriching a lipopeptide in a microbial cell culture, the method comprising mixing an amphiphilic sulfonate and / or an amphiphilic sulfate with the cell culture to induce the formation of lipopeptide-containing aggregates; centrifuging the cell culture to generate a supernatant fraction and a pellet fraction; separating the pellet fraction from the supernatant fraction; and mixing the pellet fraction with a polyoxyethylene glycol alkyl ether to release the lipopeptide from the aggregates. Also provided is a method of purifying an exopolysaccharide from a microbial cell culture, the method comprising mixing an amphiphilic sulfonate and / or an amphiphilic sulfate with the cell culture to induce aggregate formation, centrifuging the cell culture to generate a fraction. supernatant and a pellet fraction, separate the supernatant fraction from the pellet fraction, add alcohol to the supernatant fraction to precipitate the exopolysaccharide; and removing the precipitated exopolysaccharide from the supernatant fraction.