Chitosan MEMBRANES FOR USE IN CONTROLLED INSULIN RELEASE SYSTEM

摘要

Chitosan membranes for use in a controlled-release insulin system comprises a method of obtaining chitosan membranes prepared from solvent evaporation by dissolving the polymer in an acetic acid solution (1% v / v) to a final concentration of the 1% (w / v) polymer solution under magnetic stirring for a period of 24 hours and further vacuum filtration to remove insoluble substances, after which the solution is kept under magnetic stirring where, to obtain When the acid is completely neutralized, sodium hydroxide is dripped at 1m and its pH is constantly measured until neutrality is reached (ph = 7). Once the solution is formed, it is distributed in 5.5 cm petri dishes, with a final volume in each 5 ml solution plate and subjected to refrigeration at 8ºC for 5 days, for complete evaporation of the solvent. When dried, the plates are capped and the membranes kept refrigerated. As a result, the pure chitosan membrane, called membrane q, is obtained, in which insulin is incorporated using the same technique of evaporation of the plate solvent used to obtain the chitosan membrane. However, after neutralization, the The chitosan solution is distributed in petri dishes and the insulin is added to each dish so that at the end of each chitosan 5ml there is 1ml (100 æl) insulin. Insulin is slowly mixed with chitosan with the help of a stick. The plates are then placed in the refrigerator at 8 ° C for drying. Thus, the chitosan membrane with insulin, called membrane qi, is obtained.