Nucleotídicas default substitutions.

摘要

On the approaches of improving traditional plants, Chemical mutagenesis can be used to introduce substitutions nucleotÍdicas randomly in the plant genome.That is to say, without possibilities of Monitoring Sites nucleotÍdicos changes.Because of the complexities genÓmicas, Statistical probability is extremely low in finding a replacement nucleotÍdica default.The present Invention, however, demonstrates how to develop a novel use of the alternative digital polymerase chain reaction (BCPR), preferably BCPR Drops (ddpcr)To exploit the finding of specific substitutions in nucleotÍdicas mutated genes.The Platform includes a complete Survey method with a Library of mutagenizados agencies,Systems based on Digital PCR and a setup to propagate and analyze mutated organisms identified.