METHOD FOR INCREASING INTERLEUKIN-2 SECRATION LEVELS AND PROTEINS PRODUCED FROM IT

摘要

The present invention relates to the field of biotechnology, in particular to a method based on the introduction of a single mutation in the genes encoding human IL-2 and muteins derived from it, which leads to an increase in the level of secretion in different hosts without affecting their biological functions. In particular, these mutations are based on a non-conservative substitution of the amino acid at position 35 in the primary sequence of human IL-2; preferably the substitutions are K35E, K35D and K35Q. Another object of the present invention are expression systems used to produce both recombinant human IL-2 and muteins derived from it, by the method described in the present invention. The aforementioned method can be used to increase the efficiency of the production of recombinant human IL-2 and muteins derived from it both on a laboratory scale and on an industrial scale. Proteins obtained by this method can be used for therapeutic purposes, as well as for the propagation of T cells in vitro for the therapy of adaptive transfer.