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Improved methods for producing polynucleotide libraries in vaccinia virus/eukaryotic cells
Improved methods for producing polynucleotide libraries in vaccinia virus/eukaryotic cells
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机译:在牛痘病毒/真核细胞中产生多核苷酸文库的改进方法
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摘要
This disclosure provides an improved method of constructing a library of polynucleotides of interest in a poxvirus, e.g., vaccinia virus vector system, where the polynucleotides of interest encode polypeptides of interest. The method comprises constructing the library in the presence of an inhibitor of poxvirus assembly, e.g., rifampicin, which allows construction of libraries with higher complexity and diversity than previous methods.
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